2nd INTERNATIONAL TRAINING WORKSHOP
DNA MICROARRAY FOR GENE EXPRESSION
March 07 -11, 2011
CENTRE OF EXCELLENCE IN MOLECULAR BIOLOGY
Microarray Technology is finding use in gene discovery, expression profiling, comparative genomic, chromatin immunoprecipitation, SNP detection, splicing detection and pathways analysis. These techniques are being used in plant and health sciences and in clinical diagnosis. It has become important for researchers of each of these fields to be familiar with microarray technology and design experiments to get maximum information. This technique in combination with knowledge in bioinformatics can yield ample amount of information in research.
It is proposed to organize a one week course to provide theoretical background and hands-on experience in of microarray technology. This course will comprise of lectures and laboratory sessions. The course is divided into two parts:
Part I will cover wet-lab aspects of microarray e.g. experimental design, isolation of RNA, quantity and quality of RNA, printing of slides, labeling of RNA with Cy3 & Cy5, hybridization of scanned slides, image analysis Part II will cover data extraction and analysis using software; Spot finder, MIDAS and web servers KEGG, gene cards.
The course will equip participants with wet and dry laboratory techniques with regard to microarray technology, so they can design experiments in own field of research.