faculty

SS3



Dr. Sameera Hassan

Associate Professor


Plant Genomics Lab

e-mail: sameera.hassan@cemb.edu.pk

Dr. Sameera Hassan has joined CEMB, after Masters in Zoology by selection through national competition, in 1997. During her research endeavours she has worked on phage display of Bacillus thuringiensis protein where the expression of whole fragment of Cry 1 Ac toxin and domains II III were separately displayed as translational fusion with gene III in pDAN3 phagemid system. This study involved different Molecular Biological Techniques like cloning, sub-cloning, SDS PAGE analysis, Dot Blot, Western Blotting, PCR, Plasmid and Genomic DNA Isolation, Colony hybridization, DNA Sequencing and analysis through Chromas software.
Different Microbiological techniques included Streaking, colony inoculum, Glycerol stock and different media preparations.
She has been also an active researcher in Biopharmaceutical lab and worked on the expression of recombinant human erythropoietin (hEPO) gene in prokaryote by transformation in E. coli (BL21DE3) and in eukaryote by transient and stable transfection in Chinese Hamster Ovary cells. The protein was purified by using different chromatographic techniques like Affinity and ion Exchange Chromatography from cell culture supernatants.
She has also worked on multi drug resistant Staphylococcus aureus (MRDSA) from Bovine mastitis dairy samples and isolated 8MRDSA after screening through different microbiological media like Muller Hinton agar, Sheep Blood agar and Baird Parker agar.
In 2010 got enrolled in MPhil leading to PhD program. During MPhil research two desi cotton varieties were worked on for their NaCl tolerance. In 2016 her PhD was notified based on research entitled “Cellular Characterization, Cloning & Expression of Universal Stress Protein Gene (GUSP1) in Cotton and its Role in Drought Tolerance”. Since then, she is working on abiotic/biotic stress tolerant in different plants. The genes playing role in tolerance are isolated, Cloned and characterized for developing transgenic cotton plants for their respective tolerance.
Thus, two PhDs and 15 MPhils have completed their thesis, so far. A total of 10 full length genes and 8 partial cDNAs are submitted on NCBI during 2022-2023.
She is enthusiastic researcher her greatest strength is research and teaching skill which enables her to contribute permanently betterment of her institute as well as to enhance her professional skill.

Selected Publications:

  1. Batool F, Hassan S, Azam, S. et al. (2023). Transformation and expressional studies of GaZnF gene to improve drought tolerance in Gossypium hirsutum. Sci Rep 13, 5064. https://doi.org/10.1038/s41598-023-32383-0
  2. Muzaffar A, Arif U, Akram F, Batool F, Hassan S, Rashid B (2022) Abiotic Stress: Interplay Between ROS Production and Antioxidant Machinery, Signaling, and ROS Homeostasis. OBM Genetics 6 (4): doi:10.21926/obm.genet.2204171
  3. Azhar M, Batool F, Akram F, Hassan S, Rashid B (2022). Worldwide Impact of Covid-19 on Food Security and Farming Sector. Acta Scientific Biotechnology 3 (5): 01-08.
  4. Anicet B A, Sarwar M B, Jabbar B, Hassan S, Rashid B (2022). Transient Expression Analysis of Agave sisalana Heat Shock Protein Gene (AsHSP70) in Model Species (Nicotiana benthamiana) under Heat Stress Biology Bulletin 49 (3): 160-168.
  5. Hafeez MN, Khan MA, Sarwar B, Hassan S, Ali Q, Husnain T, Rashid B (2021). Mutant Gossypium universal stress protein-2 (GUSP-2) gene confers resistance to various abiotic stresses in E. coli BL-21 and CIM-496-Gossypium hirsutum. Sci Rep 11, 20466. https://doi.org/10.1038/s41598021-99900-x
  6. Hassan S, Ahmad A, Batool F, Rashid B, Husnain T (2021). Genetic Modification of Gossypium arboreum Universal Stress Protein (GUSP1) Improves Drought Tolerance in Transgenic cotton (Gossypium hirsutum). Physiology and Molecular Biology of Plants 27(8):1779-1794. https://doi.org/10.1007/s12298-021-01048-5.
  7. Sher Z, Majid B (2021). Identification, isolation and characterization of GaCyPI gene in Gossypium arboreum under cotton leaf curl virus disease stress. Phyton-International Journal of Experimental Botany. 90 (6): 1613 – 1632. https://doi.org/10.32604/phyton.2021.016154. MU, Hassan S, Batool F, Aftab B, Rashid
  8. Jabbar B, Anicet B A, Sarwar M B, Rashid B; Hassan S & Husnain T (2021). RNA-Seq Data Analysis Unveils Potential Conserved Micro-RNAs in Agave deserti. Current Proteomics 18 (2): 248-263.https://doi.org/10.2174/1570164617999200529122637.
  9. Batcho, A.A., Sarwar, M.B., Rashid, B. Hassan S, Husnain T (2021). Heat shock protein gene identified from Agave sisalana (AsHSP70) confers heat stress tolerance in transgenic cotton (Gossypium hirsutum). Theoretical and Experimental Plant Physiology. 33: 141-156. https://doi.org/10.1007/s40626-021-00200-6.
  10. Mizna, Sajid M, Hassan S, Rashid B & Husnain T (2020). Embryogenic Capabilities of Cotton through Tissue Culture and Expression Analysis of SERK3 for Regeneration Potential. Indian Journal of Agricultural Research, 54 (3): 361-366. https://doi.org/10.18805/IJARe.A-515.