Dr Noreen Latief
Stem Cells Group
Dr. Noreen initially joined the Center of Excellence in Molecular Biology (CEMB) as a Ph.D. scholar, where she pursued her doctoral studies. After successfully completing her Ph.D. degree, she joined her alma mater as an Assistant Professor in the Stem Cells Regenerative Lab. Her research endeavors are primarily centered around the ambitious objective of organ regeneration, aiming to achieve this without the necessity of organ transplantation.
Dr. Latief’s focus primarily lies in the utilization of Mesenchymal Stem Cells (MSCs) as a personalized medicine approach. Through her work, she aspires to contribute to the advancement of regenerative medicine by exploring novel strategies for organ regeneration, ultimately enhancing the quality of life for individuals affected by these challenging health issues. In pursuit of her research goals, she has chosen to address prevalent and impactful medical conditions, such as osteoarthritis and spinal cord injuries. The research group is strategically harnessing the regenerative potential of stem cells derived from various sources like Umbilical cord and adipose tissue to address the disease conditions. The primary goal here is to reinforce crucial cellular functions such as paracrine signaling, homing capability, survival mechanisms, and chemotaxis responses. This intricate modulation becomes particularly vital in the context of the demanding oxidative stress environments inherent to the targeted disease conditions.
To maximize the therapeutic impact of these interventions, the research group adopts a multifaceted approach. Stem cells are subjected to precise modulation through priming techniques, complemented by the infusion of antioxidants and phytoextracts and are seamlessly integrated with cutting-edge components like amniotic membrane, silk scaffolds and nano particles.
Dr. Noreen has international publications in esteemed journals, underscoring her significant contributions to the academic and scientific community. Additionally, her achievements include securing grants from the Higher Education Commission (HEC), further highlighting her dedication to advancing research and innovation.
Her vision encompasses the translation of fundamental research into tangible clinical applications, ultimately contributing to the betterment of society.
Areas of Expertise:
- Molecular/Recombinant DNA Techniques: PCR, Real Time PCR, Genotyping, Sequencing, Chromatography, Gel electrophoresis, Cloning Techniques, Transformation, Microtomy, Fluorescent activated cell sorting, etc.
- Biochemical Techniques: RNA and genomic DNA extraction, Elisa, Protein purification, Western blotting, Immunostaining, immunohistochemistry, etc.
- Microbiological Techniques: Disinfections, sterilization, specimen collection, smear examination, microbial cultures, biochemical examination, etc.
- Cell Culture Techniques: Umbilical cord, Bone marrow and adipose tissue derived mesenchymal stem cells culturing, chondrogenesis, transfection, etc.
- Rodent Model. Animal Model development for osteoarthritis, spinal cord injury, skin burn
Regenerative medicine for the cure of osteoarthritis (Worn out cartilage), paralysis (Spinal cord) and Amniotic membrane for the repair of ocular disorders
- Naseer N, Mustafa MM, Latief N,et al. Sarcococca salignafabricated gold nanoparticlesalleviatedin vitrooxidative stress and inflammation in humanadipose-derived stem cells.J Biomed Mater Res. 2023;1‐12.doi:10.1002/jbm.b.3530312
- F Shams, A Azeem, A Shams, A Tawab, S Rehman, S Tariq, N Latief. (2022). Flavonoid rich extract of Trigonella foenum-graecum leaves ameliorate liver fibrosis. Food Bioscience 50, 102046,ISSN 2212-4292,https://doi.org/10.1016/j.fbio.2022.102046.
- Khawaja, H., Fazal, N., Yaqub, F., Ahmad, M. R., Hanif, M., Yousaf, M. A., & Latief, N. (2021). Protective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway. PloS one, 16(10), e0258762.
- Yaqub F, Latief N, Butt H, Naseer N, Riazuddin S. Alpha lipoic acid priming enhances the hepatoprotective effect of adipose derived stem cells in CCl4 induced hepatic injury in-vitro. Eur J Pharmacol. 2021 Jun 9;906:174201. doi: 10.1016/j.ejphar.2021.174201. Epub ahead of print. PMID: 34118221.
- Ahmad MR, Badar W, Ullah Khan MA, Mahmood A, Latief N, Iqbal T, Khan Assir MZ, Sleem MA. Combination of preconditioned adipose-derived mesenchymal stem cells and platelet-rich plasma improves the repair of osteoarthritis in rat. Regen Med. 2020 Nov;15(11):2285-2295. doi: 10.2217/rme-2020-0040. Epub 2020 Dec 16. PMID: 33326341
- Fazal N, Khawaja H, Naseer N, Khan AJ, Latief N. Daphne mucronataenhances cell proliferation and protects human adipose stem cells against monosodium iodoacetate induced oxidative stress in vitro. Adipocyte.2020;9(1):495-508. doi:10.1080/21623945.2020.1812242
- Naseer N*, Bashir S*, Latief N, Latif F, Khan SN, Riazuddin S: Human amniotic membrane as differentiating matrix for in vitro chondrogenesis.Regenerative Medicine.2018 Oct 9 org/10.2217/rme-2018-0017
- Fazal N, Latief N:Bombyx mori derived scaffolds and their use in cartilage regeneration: a systematic review. Osteoarthritis Cartilage.2018 Jul 29. pii: S1063-4584(18)31384-0. doi: 10.1016/j.joca.2018.07.009
- Bhatti FU, Mehmood A,Latief N, Zahra S, Cho H, Khan SN, Riazuddin S:Vitamin E protects rat mesenchymal stem cells against hydrogen peroxide-induced oxidative stress in vitro and improves their therapeutic potential in surgically-induced rat model of osteoarthritis. Osteoarthritis Cartilage. 2017 FEB;25(2)321-331
- Latief N, Raza FA, Bhatti FU, Tarar MN, Khan SN, Riazuddin S : Adipose stem cells differentiated chondrocytes regenerate osteoarthritic cartilage. Cell Biol Int.2016 May; 40(5):579-88.